Detection, Identification and Characterization of Organisms of Pharmaceutical and Medical Significance

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Chapter: Pharmaceutical Microbiology : Bacteria

There are many situations in which microorganisms must not only be detected and enumerated but where they must also be identified either to make a specific diagnosis of infection or to ensure the absence of specified bacteria from certain types of product.


DETECTION, IDENTIFICATION AND CHARACTERIZATION OF ORGANISMS OF PHARMACEUTICAL AND MEDICAL SIGNIFICANCE

 

There are many situations in which microorganisms must not only be detected and enumerated but where they must also be identified either to make a specific diagnosis of infection or to ensure the absence of specified bacteria from certain types of product. In such circumstances various cultural approaches are available that deploy enrichment and selection media. Once a microorganism has been isolated in pure culture, usually from a single colony grown on an agar plate, then further characterization may be made by the application of microscopy together with some relatively simple biochemical tests. Over the last 20 years the biochemical characterization of individual organisms has become simplified by the introduction of rapid identification systems. In recent years molecular approaches have enabled identification of organisms without the need to culture them.

 


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