Evaluation of Solid Disinfectants

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Chapter: Pharmaceutical Microbiology : Laboratory Evaluation Of Antimicrobial Agents

Solid disinfectants usually consist of a disinfectant substance diluted by an inert powder. Phenolic substances adsorbed onto kieselguhr (diatomite) form the basis of many disinfectant powders; another widely used solid disinfectant is sodium di-chloro-isocyanurate.


EVALUATION OF SOLID DISINFECTANTS

 

Solid disinfectants usually consist of a disinfectant substance diluted by an inert powder. Phenolic substances adsorbed onto kieselguhr (diatomite) form the basis of many disinfectant powders; another widely used solid disinfectant is sodium di-chloro-isocyanurate. Other disinfectant or antiseptic powders used in medicine include acriflavine and compounds with antifungal activity such as zinc undecenoate or salicylic acid mixed with talc. These disinfectants may be evaluated by applying them to suitable test organisms growing on a solid agar medium. Discs may be cut from the agar and sub-cultured for enumeration of survivors. Inhibitory activity is evaluated by dusting the powders onto the surface of seeded agar plates, using the inert diluents as a control. The extent of growth is then observed following incubation.

 

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