Culture Techniques - Bacteria

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Chapter: Pharmaceutical Microbiology : Bacteria

Conventional approaches to microbiological examination of specimens require that they be cultured to assess the total numbers of specific groups of microorganisms or to determine the presence or absence of particular named species.


CULTURE TECHNIQUES

 

Conventional approaches to microbiological examination of specimens require that they be cultured to assess the total numbers of specific groups of microorganisms or to determine the presence or absence of particular named species. The majority of samples taken for examination contain mixtures of different species, so simple plating onto an agar surface may fail to detect an organism that is present at <2% of the total viable population. Various enrichment culture techniques may therefore be deployed to detect trace numbers of particular pathogens, prior to confirmatory identification.

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