Detection, Identification and Characterization of Organisms of Pharmaceutical and Medical Significance
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There are many situations in which microorganisms must not only be detected and enumerated but where they must also be identified either to make a specific diagnosis of infection or to ensure the absence of specified bacteria from certain types of product.
DETECTION, IDENTIFICATION AND CHARACTERIZATION OF ORGANISMS OF
PHARMACEUTICAL AND MEDICAL SIGNIFICANCE
There are many
situations in which microorganisms must not only be detected and enumerated but
where they must also be identified either to make a specific diagnosis of
infection or to ensure the absence of specified bacteria from certain types of
product. In such circumstances various cultural approaches are available that
deploy enrichment and selection media. Once a microorganism has been isolated
in pure culture, usually from a single colony grown on an agar plate, then
further characterization may be made by the application of microscopy together
with some relatively simple biochemical tests. Over the last 20 years the
biochemical characterization of individual organisms has become simplified by
the introduction of rapid identification systems. In recent years molecular
approaches have enabled identification of organisms without the need to culture
them.
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