Microscopy of Organisms of Pharmaceutical and Medical Significance

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Chapter: Pharmaceutical Microbiology : Bacteria

Observation of stained and wet preparations of clinical specimens (blood, pus, sputum) and isolated pure cultures of bacteria from the manufacturing environment provides rapid and essential information to guide further identification.


MICROSCOPY OF ORGANISMS OF PHARMACEUTICAL AND MEDICAL SIGNIFICANCE

 

 

Observation of stained and wet preparations of clinical specimens (blood, pus, sputum) and isolated pure cultures of bacteria from the manufacturing environment provides rapid and essential information to guide further identification. The application of simple stains such as the Gram stain can divide the various genera of bacteria into two convenient broad groups. The size and shapes of individual cells and their arrangement into clusters, chains and tetrads will also guide identification, as will specific stains for the presence of endospores, capsules, flagella and inclusion bodies. Examination of wet preparations can give an indication as to the motility status of the isolate, and these procedures all represent an important first stage in the identification process.

 

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