Determination of Protein-Drug Binding
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The analytical techniques used in protein binding studies can be divided into two classes
DETERMINATION OF PROTEIN-DRUG BINDING
The analytical techniques used in protein binding
studies can be divided into two classes –
1. Indirect techniques are those based on the separation of bound form from the free micromolecule.
These techniques are usually applied in biological samples (blood, serum,
plasma) for the determination of the percentage of binding. Indirect analytical
methods used in protein binding studies are equilibrium dialysis, dynamic
dialysis, ultrafiltration, diafiltration, ultracentrifugation, gel filtration,
etc.
2. Direct techniques are those that do not require the separation of bound form of drug from
the free micromolecule. These methods are used for the estimation of the number
and the elucidation of the character of binding sites in pure aqueous solutions
of proteins. Direct techniques used in protein binding studies are UV
spectroscopy, fluorimetry, and ion-selective electrodes.
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