Determination of Protein-Drug Binding

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Chapter: Biopharmaceutics and Pharmacokinetics : Protein Binding of Drugs

The analytical techniques used in protein binding studies can be divided into two classes


DETERMINATION OF PROTEIN-DRUG BINDING

The analytical techniques used in protein binding studies can be divided into two classes –

1. Indirect techniques are those based on the separation of bound form from the free micromolecule. These techniques are usually applied in biological samples (blood, serum, plasma) for the determination of the percentage of binding. Indirect analytical methods used in protein binding studies are equilibrium dialysis, dynamic dialysis, ultrafiltration, diafiltration, ultracentrifugation, gel filtration, etc.

2. Direct techniques are those that do not require the separation of bound form of drug from the free micromolecule. These methods are used for the estimation of the number and the elucidation of the character of binding sites in pure aqueous solutions of proteins. Direct techniques used in protein binding studies are UV spectroscopy, fluorimetry, and ion-selective electrodes.

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